不同植物药提取物对灵芝细胞生长和胞内三萜产物形成的影响

Effects of extracts from different phytomedicines on cell growth and intracellular triterpenoids formation of Ganoderma lucidum in submerged fermentation

研究了不同植物药的水提和醇提物对灵芝深层发酵过程中菌丝量和胞内三萜产量的影响。将不同植物药的水提物和醇提物分别加入到发酵基础培养基中,培养7d后检测灵芝生物量和胞内三萜含量。结果表明,金银花和枸杞子水提物添加浓度为100mg/L时,可促进灵芝细胞的生长(p<0.05)。连翘水提物对灵芝生长和胞内三萜的形成都有显著促进作用,当连翘水提物浓度为400mg/L时,胞内三萜产量从对照的(192.54±8.99)mg/L提高到(302.52±3.79)mg/L。金银花和枸杞子醇提物浓度为200mg/L时能显著促进灵芝细胞生长;枸杞子醇提物在同样浓度下还能促进灵芝胞内三萜的形成。但板蓝根和银杏叶水提物和醇提物都对灵芝的细胞生长和胞内三萜形成有较强的抑制作用。

The effects of water and ethanol extracts from different phytomedicines on mycelial growth and intracellular triterpenoids（ITS） formation of Ganoderma lucidum in submerged fermentation were studied.The medicinal fungus G.lucidum was inoculated into the basal media with and without addition of extract of phytomedicines,and then cultured for 7d.The results showed that both water extracts from Lonicera japonica and Lycium chinensis at 100mg/L increased mycelial biomass production of G.lucidium（p0.05）;while the water extract from Forsythia suspensa had significantly stimulatory effects on both the mycelial growth and ITS production,and the ITS yield increased from（192.54±8.99）mg/L to（302.52±3.79）mg/L when the concentration of the water extract was 400mg/L.In addition,ethanol extracts from L.japonica and L.chinensis at 200mg/L showed positive effects on the mycelial growth,and the ethanol extract from L.chinensis displayed stimulatory effects on ITS production.However,both the water extracts and ethanol extracts from Isatis tinctoria and Ginkgo biloba showed the inhibitory effects on the mycelial growth and ITS production of G.lucidium.