妊娠绵羊子宫内膜组织内源性反转录病毒及其受体HYAL2基因表达水平的检测

Detection of expression level of endogenous jaagsiekte sheep retrovirus and its receptor HYAL2 in endometrium of pregnant Mongolian sheep by TaqMan real-time PCR and in situ hybridization

为了探究内源性反转录病毒(enJSRV)及其受体HYAL2在妊娠蒙古绵羊子宫内膜形成和发育过程中的作用,运用TaqMan实时荧光定量PCR和组织原位杂交技术对其在不同妊娠时期(30、50、70、90、110和130d)蒙古绵羊子宫内膜中的表达规律和分布定位进行了研究。实时荧光定量PCR结果显示,enJS-RV及其受体HYAL2在蒙古绵羊子宫内膜的不同妊娠时期均有表达,通过SPSS统计学软件分析可以看出,妊娠蒙古绵羊子宫内膜组织中enJSRV mRNA的表达于妊娠第30和50天相对较高,70～130d均低于对照组(30d),且差异都极显著,enJSRV与其受体HYAL2mRNA之间亦无线性相关性。原位杂交结果显示,enJSRV及其受体HYAL2mRNA在妊娠第30、50和130天蒙古绵羊子宫内膜的腔上皮细胞、腺上皮细胞、血管内皮细胞、间质及滋养层巨型双核细胞中均有阳性信号表达。表明enJSRV及其受体HYAL2表达于上皮细胞及上皮细胞来源的滋养层巨型双核细胞中,揭示enJSRV及其受体HYAL2在子宫形成过程中和系统防御中可能发挥重要作用。

In order to assess the expression of endogenous jaagsiekte sheep retrovirus（enJSRV） and its receptor HYAL2 mRNAs in the endometrium of Mongolian ewes throughout gestation,by fluorescent quantitative RT-PCR,enJSRV and HYAL2 mRNAs were found to be expressed throughout gestation in the endometrium.The mRNA expression level of enJSRV was higher in placenta on day 30 and 50 during pregnancy.The in situ hybridization results showed that enJSRV and HYAL2 mRNAs were specifically expressed in endometrial luminal epithelium and glandular epithelium,endometrial caruncle,trophoblast giant binucleate cells,as well as stroma,and blood vessel endothelial cells endometrial caruncle,placental cotyledon,stroma and trophectoderm.Collectively,little was known about the molecular mechanisms that trophoblast differentiation and multinucleated syncytia formation regulated by enJSRV,however the temporal and spatial reconstitutions of enJSRV expression in the endometrium of Mongolian ewes indicated that enJSRV and its receptor HYAL2 were beneficial to the host and were involved in protection of the uterus from viral infection and regulators of placental morphogenesis and function.