双光子技术及其在生物医药分析中的应用

Two-photon techniques and their applications in biomedical and pharmaceutical analysis

作为近20年来迅速发展的一门新技术,双光子技术已被广泛应用于三维数据存储材料、医药、军事、生物以及生命科学等领域,并随学科交叉与融合,在揭示生命活动基本规律和起源的研究中发挥越来越重要的作用,为生物医学提供了更多、更为有效的手段.本文从常用的双光子材料以及双光子激光扫描荧光显微镜成像原理等方面,结合双光子技术在生物医学研究中的应用,探讨了这一新兴技术在生物医药分析领域的前景.

Two-photon techniques, which have undergone rapid development during the last two decades, have attracted a great deal of attention from scientists and researchers in many fields. This has led to a wide range of applications in three-dimensional data storage, medicine, defense, biology, and life sciences. At the same time, there has been increased integration and cooperation among various disciplines. Two-photon techniques have increasingly important roles in revealing the basic principles and origins of life activities, and provide a much more effective means for developing biological and medical imaging. Combining the history of the two-photon technique with its applications in the life sciences, this mini-review provides a simple introduction to these emerging technologies. It focuses on the development of two-photon techniques, two-photon materials used in scientific research and their applications in optical and medical research, and the imaging principles of the two-photon laser scanning fluorescent microscope.