肿瘤M2型丙酮酸激酶在结肠癌诊断中的意义

The value of tumor M2 pyruvate kinase in diagnosis of colon cancer

目的评估肿瘤M2型丙酮酸激酶(M2-PK)在结肠癌的诊断、筛查以及肿瘤分期中的意义。方法用ELISA检测结肠癌33例,结肠腺瘤27例以及正常对照30例的血浆及粪便中肿瘤M2-PK的数值,同时用化学发光法(CL)检测结肠癌患者癌胚抗原(CEA)的水平,并进行相关分析。结果结肠癌组血浆及粪便肿瘤M2-PK水平显著高于结肠腺瘤组及正常对照组(P<0.05),但结肠腺瘤组与正常对照组差异无统计学意义(P>0.05)。结肠癌Dukes C、D期患者的血浆及粪便中肿瘤M2-PK水平明显高于A、B期患者(P<0.05),有淋巴转移者明显高于无淋巴转移者(P<0.05)。血浆和粪便肿瘤M2-PK对结肠癌诊断的敏感性分别为84.8%和78.8%,高于CEA的51.5%。结论肿瘤M2-PK对结肠癌的诊断具有临床价值,敏感性高于CEA,并且与肿瘤的分期密切相关,可用于结肠癌的筛查及诊断。

Objective To evaluate the value of tumor M2 pyruvate kinase（M2-PK） in diagnosing,screening and staging colon cancer.Methods The levels of tumor M2-PK in plasma and fecal were determined by ELISA in 33 patients with colon cancer（group A）,27 patients with colon polyp（group B） and 30 healthy controls（group C）.Dukes staging of the tumors was performed as well.Carcinoembryonic antigen（CEA） was simultaneously determined by chemiluminescence in group A.Results The level of tumor M2-PK was significantly higher in group A than that in groups of B and C（P0.05）,which was not significantly different between groups of B and C（P0.05）.The levels of tumor M2-PK were higher in cases with Dukes staging C and D than those with Dukes staging A and B（P0.05）.The level of tumor M2-PK was higher in patients with lymph node metastasis than that without（P0.05）.The sensitivity of tumor M2-PK in plasma and fecal was 84.8% and 78.8%,respectively,which was significantly higher than 51.5% for that of CEA.Conclusion Tumor M2-PK in fecal and plasma has clinical value in diagnosis of colon cancer.The sensitivity of tumor M2-PK for colon cancer is higher than that of CEA.Tumor M2-PK is correlated to the stage of colon cancer and may be helpful for screening and diagnosing colon cancer.