纤溶酶原激活物抑制剂1在肝纤维化组织中的表达及意义

Expression and significance of plasminogen activator inhibitor-1 in hepatic fibrosis

目的探讨纤溶酶原激活物抑制剂1（PAI-1）与肝纤维化发生的关系。方法对30例乙型肝炎患者行肝脏活检,明确肝纤维化分级。正常肝组织3例,1~2级肝纤维化12例,3级肝纤维化8例,肝硬化7例。采用免疫组化及半定量分析方法,检测PAI-1在肝纤维化组织中的表达。结果在正常肝脏中,PAI-1仅在汇管区细胞浆内有少量表达;肝纤维化组PAI-1表达均高于正常肝组织（P〈0.05）,且随纤维化程度而加重（P〈0.05）。在纤维化肝脏,PAI-1主要分布于细胞浆。结论 PAI-1在肝纤维化组织中高表达可能在肝纤维化的发生中起重要作用。

Objective To investigate the relationship and significance between expression of plasminogen activator inhibitor-1（PAI-1） and hepatic fibrosis.Methods On the basis of liver biopsy,20 patients with hepatitis B were classified as normal liver tissues in 3 cases,hepatic fibrosis grade 1 to 2 in 12 cases,grade 3 in 8 cases,and liver cirrhosis in 7 cases.PAI-1 expression in the liver tissues was detected by immunohistochemistry and semi-quantitative method.Results There was only weak expression of PAI-1 in normal liver.The expression of PAI-1 was significantly higher in the tissues with hepatic fibrosis（P0.05）,which increased accordingly with severity of fibrosis（P0.05） and mainly located within endochylema.Conclusion Overexpression of PAI-1 in fibrotic liver may play an important role in the pathogenesis of hepatic fibrosis.