艾灸预处理对大负荷训练运动员Th1/Th2失衡的调节作用

Regulating effect of moxibustion pretreatment on Th1/Th2 imbalance of athlets during course of heavy load training

目的:探讨艾灸预处理对大负荷训练期间运动员身体免疫机能的影响。方法:12名中长跑男子运动员,随机分为空白组(6名)和艾灸组(6名),艾灸组在训练时采用艾灸关元、足三里和关元、三阴交,每日交替施灸,连续4周;空白组只训练不予任何干预。分别在运动训练前、运动训练3周后、调整训练1周后,采用实时荧光定量PCR检测外周血γ-干扰素(IFN-γ)和白介素-4(IL-4)细胞因子mRNA的表达量。结果:①大负荷训练3周后,血睾酮/血皮质醇(T/C)比值空白组为0.017±0.003、艾灸组为0.021±0.007,分别与同组大负荷训练前(0.036±0.009、0.037±0.013)相比,T/C比值明显降低(均P<0.01);②大负荷训练3周后,空白组IFN-γ mRNA(2.76±1.28)和IFN-γ mRNA/IL-4 mRNA比值(3.28±1.76)明显高于同组大负荷训练前(1.75±0.44、1.52±0.76)(均P<0.05);调整训练1周后,空白组IFN-γmRNA/IL-4 mRNA比值(0.86±0.66)明显低于同组大负荷训练后(3.28±1.76)(P<0.05);③艾灸组运动员IFN-γ mRNA、IL-4 mRNA及IFN-γ mRNA/IL-4 mRNA比值随时间的变化先上升,后恢复,但3次之间差异无统计学意义(均P>0.05)。结论:艾灸预处理方法可预防大负荷训练期间中长跑男子运动员Th1/Th2失衡。

Objective To explore the effect of moxibustion pretreatment on the immune function of athletes during the courses of heavy load training. Methods Twelve male middle-distance race athletes were randomly divided into an observation group （n=6） and a control group （n= 6）. In observation group,mild moxibustion was applied at Guanyuan （CV 4）, Zusanli （ST 36） or Guanyuan （CV 4）, Sanyinjiao （SP 6） alternatively once daily, for 4 weeks. In control group, there was no any intervention. The expressions of γ-interferon （IFN-γ） and interleukin-4 （IL-4） were detected by reahime fluorescent quantitative PCR method before heavy load training, after 3 weeks of treatment, after 1 week adjusting training. Results （1）The ratios of Testosterone/Cortisol （T/C） of （0. 017±0. 003） in the control group and of （0. 021±0. 007） in the observation group after heavy load training for three weeks were significant lower than 0. 036±0. 009 and 0. 037±0. 013 before heavy load training in the same group, respectively （both P〈0.01） （2）IFN-γ mRNA of （2. 76±1. 28） and ratio of IFN-γ mRNA/IL-4 mRNA of （3.28±1.76） in the control group after heavy load training for three weeks were significant higher than 1.75 ± 0.44 and 1.52 ± 0.76 before heavy load training, respectively （both P〈0.05）, and ratio of IFN-γ mRNA/IL-4 mRNA of （0. 86±0. 66） after adjusting training for 1 week reduced significantly as compared with that after heavy load training for three weeks （P〈0.05）. （3）In the observation group, IFN-ymRNA, IL-4 mRNA and ratio of IFN-γ mRNA/IL-4 mRNA increased firstly and then restore with the time, but these changes had no significant differences （all P〉0.05）. Conclusion Moxibustion pretreatment can prevent Th1/Th2 imbalance of male middle-distance race athlete during the course of heavy load training.