慢性非细菌性前列腺炎SD大鼠前列腺平滑肌细胞内钙离子浓度的变化

Intracellular calcium ion concentration in the prostate smooth muscle cells of chronic abacterial prostatitis rat models and normal controls

目的:探讨SD大鼠慢性非细菌性前列腺炎(CAP)炎症组与正常对照组前列腺平滑肌细胞(PSMCs)内游离钙离子浓度([Ca2+]i)的差异及其在CAP中的意义。方法:利用SD大鼠前列腺蛋白提取液与弗氏完全佐剂(FCA)诱导出CAP模型,体外贴壁培养并纯化炎症组与正常对照组PSMCs,细胞经钙离子荧光探针FLUO-3AM孵育后在激光共聚焦显微镜(LSCM)下连续动态扫描。结果:CAP组与对照组的PSMCs钙离子荧光强度分别为80.39±9.00和27.95±10.04,差异具有显著性(P<0.01)。结论:CAP SD大鼠PSMCs[Ca2+]i明显升高,其升高可能增强了PSMCs的收缩作用,从而引起CAP的一系列如对疼痛敏感性增强、下腹部疼痛不适的症状。

Objective：To investigate the difference in intracellular calcium ion i concentration in prostate smooth muscle cells（PSMCs） between SD rat models of chronic abacterial prostatitis（CAP） and normal controls,and to evaluate the role of i concentration in CAP.Methods：We established CAP models in SD rats using purified prostate protein and Freund＇s complete adjuvant,cultured in vitro and then purified the PSMCs of both the CAP models and normal controls.Continuous dynamic scanning was performed under the laser confocal scanning microscope after incubation of the cells with FLUO-3AM.Results：The fluorescence intensities of in the PSMCs were 80.39±9.00 and 27.95±10.04 in the CAP models and normal controls,respectively,with statistically significant differences between the two groups（P0.01）.Conclusion：The concentration of i increased in the PSMCs of the CAP rat models,which might enhance the constriction of PSMCs and subsequently increase the sensibility to pain and cause lower abdominal pain.