氧化铝气体分布器应用小球藻培养的研究

The Effect of Aeration on the Growth and Lipid Content on Chlorella vulgaris LICME002 in the Applied Alumina Gas Distributor Bubbling Column Photobioreactor

光生物反应器设计中,气体分布器对微藻生长有较大的影响,尤其是在鼓泡式光生物反应器中更为显著。实验考察了采用氧化铝烧制的多孔气体分布器的5L鼓泡式光生物反应器中通气速率、CO2浓度对小球藻LICME002生物量、叶绿素含量、油脂积累的影响。对该气体分布器下的CO2浓度和通气速率对小球藻的作用机理进行了初步的探讨。结果表明,CO2浓度为3%时,该株微藻生物量、叶绿素、油脂积累的最佳;CO2浓度超过6%时各项指标显著下降。通过对0.1vvm,0.4vvm,0.7vvm、1.0vvm的通气速条件下的各项指标的分析,确定最佳通气条件为0.4vvm。结论显示,在最佳通气速率和CO2浓度下,微藻生物量能达到1.52g/L,油脂含量达到31.5%。

In the study of photobioreactor,gas distributor has great influence to the growth of microalgae,especially in the bubbling column reactor.The effect of the gas-flow rate and CO2 concentration on the biomass,chlorophyll a,and lipid accumulation of Chlorella vulgaris LICME002 in the 5L bubbling photobioreactor with a alumina gas distributor.The results showed that the 3% CO2 is the optimum condition for biomass,chlorophyll a,oil accumulation.When the CO2 concentration exceeded 6%,the algae＇s parameters decreased significantly.With the analysis of the algae＇s parameters at 0.1vvm,0.4vvm,0.7vvm,1.0vvm,and the gas-flow rate 0.4vvm is the best one.Results showed that the optimum gas-flow rate and CO2 concentration,the microalgae biomass can achieve 1.52 g/L,oil content achieved 31.5%.