螺内酯抑制大鼠角膜碱烧伤新生血管形成的作用

Inhibitory effects of spironolactone on corneal neovascularization induced by alkali burn in rats

目的:观察螺内酯(spironolactone,SL)对大鼠角膜碱烧伤后角膜新生血管(corneal neovascularization,CRNV)形成的抑制作用。方法:健康SD大鼠42只随机分为3组,6只6眼为正常组,其余36只36眼建立碱烧伤诱导的大鼠CRNV模型后随机分为实验组和对照组,每组18只。实验组给予SL(100mg/kg)灌胃,对照组给予等量生理盐水灌胃,1次/d。术后第4,7,14d在裂隙灯显微镜下观察CRNV的面积。结果:对照组在碱烧伤后4d,大鼠角巩膜缘处有新生血管芽成刷状生长,生长速度迅速,至14d新生血管交织成网状,几乎布满整个角膜。实验组大鼠CRNV管径细、分布生长速度缓慢。在碱烧伤后4,7和14d实验组CRNV面积均明显小于对照组(P<0.01)。

AIM：To obtain the inhibititive effects of spironolactone（SL）on corneal neovascularization（CRNV）induced by alkali burn in rats.METHODS：Totally 42 SD rats were divided into three groups randomly,6 rats were in normal group;CRNV models induced by alkali burn were established on the other 36 SD rats,which were randomly divided into study group and control group,18 in each group.Study group was treated orally with SL（100mg/kg）and control group was treated with the same volume of normal saline,once a day by gastric intubation.The area of CRNV was observed and calculated by slit-lamp microscope at the 4th,7th and 14th day.RESULTS：The rats of control group appeared new vascular buds on 4th day,its growth velocity was quick and became a net on the 14 day.The CRNV of study group was smaller and its growth velocity was slow.Compared with control group,the CRNV area of study group was significant smaller（P〈0.05）.CONCLUSION：SL can inhibit the occurrence of CRNV.