摘要
目的:观察形觉剥夺性近视豚鼠巩膜组织学改变和TGF-β2在巩膜中的表达。方法:用头套形觉剥夺右眼的方法诱导近视动物模型,实验前后检测豚鼠双眼屈光状态及眼轴长度。HE染色观察后极部巩膜形态学改变,免疫组织化学法对TGF-β2在巩膜内的表达进行定性及半定量测定。结果:实验组巩膜变薄、胶原纤维排列紊乱,粗细不均,纤维间隙变大,纤维走向不一,板层结构不清;巩膜TGF-β2表达明显下调。结论:形觉剥夺性近视眼的巩膜产生一系列的退行性改变,TGF-β2是形觉剥夺性近视形成的重要因素之一。
AIM：To observe the structure changes of sclera and determine the expression of TGF-β2 in the sclera of the model.METHODS：A facemask was worn on the right eyes of guinea pigs to develop form deprivation myopia.Before and after experiment,refraction was measured using retinoscope,and the ocular axial was determined by A-scan ultrasonography.Morphologic alterations of the posterior sclera were observed by light microsope.The expression of TGF-β2 in the posterior sclera was determined by immunohistochemistry.RESULTS：Posterior sclera of myopic eyes became thinner,and collagen fibres were disordered,fibers diameters were dissimilarity,the space between the fibres was larger,the parallel structure was illegible.The immunohistochemisty showed that expression of TGF-β2 in model eyes were lower than control eyes（P〈0.05）.CONCLUSION：Covering eyes with facemasks is effective,simple and convenient in making myopic model.The sclera of the model eye appeared degeneration.TGF-β2 plays an important role in the course of development in form deprivation myopia.
出处
《国际眼科杂志》
CAS
2011年第4期602-604,共3页
International Eye Science
