温度对茄病镰刀菌生长情况及产孢量的影响

Effect of temperature on the growth and spore production of Fusarium solani

目的探讨不同孵育温度对茄病镰刀菌生长状态的影响,为获得大量孢子寻找较好方案。方法茄病镰刀菌按孵育温度不同分为6组,A组、B组、C组、D组、E组分别于20℃、25℃、30℃、35℃、37℃温箱黑暗孵育7 d,F组于35℃温箱黑暗孵育3 d再于25℃温箱黑暗孵育至7 d,各组在培养24 h、3 d、5 d、7 d时分别用游标卡尺测量各组菌落直径,在培养3d、5 d、7 d时用血平板计数器计数产孢量。结果在20～30℃范围内茄病镰刀菌菌落生长速度随温度升高而增快,在30～37℃范围内菌落生长速度随温度升高而减慢,F组产孢量较多(为1.025×108 CFU/mL),而菌丝相对较少。结论茄病镰刀菌在20～37℃范围内均可以生长,30℃较适合茄病镰刀菌菌落生长,35℃温箱孵育3 d再于25℃温箱孵育至7 d是获得大量孢子而非菌丝的较好方案。

Objective To investigate the influence of temperature on the growth,sporulation quantity and pathogenicity of Fusarium solani.To find out the best way for promoting conidial production of Fusarium solani.Methods Fusarium solani from 5 different groups were incubated in dark for 7 days at 20℃,25℃,30℃,35℃,and 37℃,respectively.Group F was incubated in dark for 3 days at 35℃,and at 25℃ for the next 4 days.The diameters of the colonies were measured by vernier caliper on 24 h,3 d,5 d,and 7 d.Conidial production was determined by hematimeter on 3 d,5 d,and 7 d.Results Between The growing speed of Fusarium solani colony increased during 20℃ to 30℃,while decreased during 30℃ to 37℃ with the temperature going up.Conclusions Fusarium solani began to grow and produced conidiospore at 20 to 35℃.The pathogenicity of the Fusarium solani was affected by temperature obviously,with 30℃ as the suitable temperature.Incubation at 35℃ for 3 days and at 25℃ for consecutive 4 days was good for sporulation.