甘蔗宿根矮化病研究进展

Research advances in ratoon stunting disease of sugarcane

甘蔗宿根矮化病(Ratoon stunting disease,RSD)是由一种木质部限制性病原菌引起的病害,感染RSD的蔗株一般表现为植株矮化、分蘖少、生长缓慢,成熟蔗茎基部节位维管束组织一般呈粉红色至橙红色等变色症状。被染污的收割工具很容易将病菌传染给健康蔗株而使RSD迅速传播蔓延。酶免疫方法和PCR方法是检测RSD的常用方法,各有优缺点。用于RSD病原细菌培养的培养基有多种,使用最多的是SC培养基。选育抗RSD品种和健康种茎生产体系相结合是防治RSD最有效的措施。对RSD的基因分析已有一定进展,建议进一步利用基因组学和蛋白质组学分析方法进行研究。

Ratoon stunting disease （RSD） of sugarcane is caused by a xylem limiting pathogen Leifsonia xyli subsp. xyli （Lxx）, the plants infected with RSD are characterized by poor growth, dwarf stalks, lesser tillers. Pinkish to orange red color could be observed in the vascular bundle tissue in lower internodes of the matured sugarcane stalk. The RSD pathogen easily transferred to the healthy cane with infected harvesting tools and makes it spread rapidly. The PCR and enzyme immunoassay methods are most common for the detection of RSD in cane stalk and each of them has advantages and limits. Several culture media have been used for culturing the pathogen, of which the SC medium is the most common. The most effective way to control RSD is the use of resistant varieties combined with the use of pathogen free healthy seedcane. Some progresses have been made to explore RSD pathogen and affected plant to get the information at gene level. Genomics and proteomics approaches have been suggested to explore the plant pathogen relationship in order to control the disease.