地高辛标记cRNA探针原位杂交检测大鼠脑NMDAR-LmRNA

Detection of NMDAR-L mRNA in Rat Brain by in situ Hybridization Using Digoxin Labelled cRNA Probe

采用地高辛（Ｄｉｇｏｘｉｎ Ｄｉｇ）标记神经递质受体ＮＭＤＡＲＬｃＲＮＡ探针技术进行原位杂交，研究了Ｗｉｓｔａｒ 新生大鼠脑神经细胞ＮＭＤＡＲＬ的基因表达．光学显微镜观察结果显示，大脑皮层、海马等区有明显的ＮＭＤＡＲＬｍＲＮＡ表达，胞浆着蓝色，胞核不着色，背景浅淡，反差显著．实验结果表明地高辛标记ｃＲＮＡ探针能快速、准确地检测出ＮＭＤＡＲＬｍＲＮＡ，为进一步研究这一拟受体在脑中表达和分布奠定了基础．

Expression of NMDAR L mRNA in the brain of newborn wistar rats was detected by in situ hybridization using digoxin labelled cRNA probe of the neurontransmiter receptor (NMDAR L). The results showed that expression of NMDRA L mRNA was mainly present in the cytoplasm of cerebral cortex and hippocampus but not in nucleus with low background and better contrast. These results suggest that Digoxin labelled cRNA could be a good probe to detect the expression of NMDAR L mRNA rapidly and precisely.