诺丽(Morinda citrifolia Linn.)离体培养研究

STUDY ON CULTURE IN VITRO OF NONI(MORINDA CITRIFOLIA LINN.)

为诺丽遗传转化奠定基础及诺丽工厂化育苗提供依据,本实验以诺丽茎尖及带芽茎段为外植体,以3%MS培养基添加不同的激素组合进行离体培养,选择最佳的培养组合。实验结果表明,茎尖在MS或添加NAA(0.3 mg/L)的MS培养基中均可得到完整植株,且在后者中的长势更好。该研究成功建立了诺丽离体再生体系,为规模化生产及遗传转化提供了技术支撑。

To provided a theory foundation of genetic transformation and industrialized breeding of noni（Morinda citrifolia Linn.）,the perfect regeneration system was established using shoot tip and stem with buds of noni as explants in MS culture medium with different hormones combinations.The result showed that the complete plantlet was obtained by shoot tip cultured in MS or MS＋NAA（0.3 mg/L）,and the growth vigour was more better in the latter medium.The study established the regeneration system of noni,and provided a technology foundation for large scale production.