血府逐瘀汤对抑制大鼠动脉粥样硬化形成的作用效果研究

THE EFFECT OF XUEFU ZHUYU DECOCTION ON ATTENUATION OF THE ATHEROSCLEROSIS IN THE RATS WITH ATHEROSCLEROSIS

研究血府逐瘀汤对动脉粥样硬化(Atherosclerosis,AS)大鼠血脂及主动脉内膜粥样硬化病变的影响。将40只SD大鼠随机分为对照组、模型组、治疗组(血脂康组、血府逐瘀汤高剂量组、血府逐瘀汤低剂量组),每组8只。其中对照组给予正常饮食,模型组给予高脂饮食,治疗组在高脂饮食的基础上分别给予血脂康、高剂量血府逐瘀汤、低剂量血府逐瘀汤干预,12周后各组动物取主动脉起始部做病理学切片,进行光镜及电镜观察。腹主动脉取血并分离血清,检测其血清胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL-c)、高密度脂蛋白水平(HDL-c)。研究结果发现血府逐瘀汤高、低剂量组大鼠的主动脉光镜及电镜结构病变均比模型组轻。血府逐瘀汤高、低剂量组能明显降低大鼠血清中TC、TG和LDL-c水平,升高HDL-c水平(P<0.01)。血府逐瘀汤可有效阻止动脉粥样硬化病变的发生,其机制可能与其有效调节血脂水平的作用有关。

To study the effect of Xuefu Zhuyu decoction on attenuation of the atherosclerosis in the rats with atherosclerosis.Forty Sprague Dawley rats were divided randomly into groups of 8 rats each,control group,model group,and three treatment groups（Xue Zhi Kang group,high dose Xuefu Zhuyu decoction group,and low dose Xuefu Zhuyu decoction group）.The control group was fed with normal diet,model group a high-fat diet,treatment groups the high fat diet mixed with Xue Zhi Kang,High dose and low dose Xuefu Zhuyu decoction respectively.After 12 weeks,treatment,the morphological characteristics（HE stain and ultrastructure） of the aorta were observsed,the blood were taken from the abdominal aorta,and the blood levels of cholesterol（TC）,triglycerides（TG）,low-density lipoprotein（LDL-c）,and high-density lipoprotein（HDL-c） were detected later.It was found that the aortic morphological changes of both the high and dose Xuefu Zhuyu decoction group are lighter than that of model group.The Xuefu Zhuyu decoction groups could significantly decrease the blood level of TC,TG,and LDL-c,increasing that of HDL-c（P0.01）.So Xuefu Zhuyu decoction can attenuate the atherosclerosis effectively,whose pathogenesis might be related with its adjusting role on the blood level of lipid.