多胎与单胎山羊发情期卵巢褪黑素受体基因的克隆测序分析

SEQUENCING ANALYSIS OF MELATONIN RECEPTOR IN OVARY OF GOAT

为揭示褪黑素影响山羊繁殖性状的遗传机制,本研究采用差异显示PCR(DDRT-PCR)和克隆测序技术,研究了褪黑素受体1a(MelR1a)基因在多胎的济宁青山羊和单胎的沂蒙黑山羊发情期卵巢组织中的表达情况,并对MelR1a基因进行了克隆测序分析。检测结果表明,MelR1a在两种山羊卵巢中均有表达,说明,褪黑素可以通过分布于卵巢中的褪黑素受体而对山羊的生殖发挥重要的调控作用。克隆测序分析结果表明,两种山羊发情期卵巢组织中褪黑素受体1a基因的碱基组成没有变异,说明山羊褪黑素受体基因序列具有高度的保守性。

In order to clarify the genetic mechanisms of melatonin affected goat reproduction traits,differential display PCR,cloning and sequencing techniques were used to detected the expressions of melatonin receptors（MelR1a） in ovary tissure of Jining Gray Goat and Yimeng Black Goat in oestrus stage,while MelR1a genes from the two kinds of goats were cloning and sequencing.The results showed that MelR1a genes were expressed in ovary tissues of the both goats,and the bases sequences of MelR1a genes were the same.The results suggest that MelR1a genes expressed in both goat ovaries play an important role in controlling the reproduction traits obviously,and make clear that Mel1a is conservative.