深圳市外来劳务工乙型肝炎血清学检测分析

Serological survey of hepatitis B virus infection among out-labor workers in Shenzhen

目的了解2007~2009年深圳市外来劳务工乙型肝炎感染模式和状况,探讨乙肝5项和HBV DNA检出率之间的关系,为深圳市劳务工群体乙型肝炎的预防和控制提供科学依据。方法利用酶联免疫吸附法（ELISA）和实时荧光定量PCR法对2007~2009年1 226份深圳市外来劳务工的血清标本进行乙肝5项检测和HBV-DNA测定。结果深圳市外来劳务工乙肝5项异常者的HBV DNA总阳性率为48.94%。共发现7种HBV感染模式,小三阳患者占HBV感染总例数的49.67%,大小三阳HBV DNA的检出率分别为94.11%和21.67%。20~30岁的青年劳务工乙肝感染人数在A-G感染模式中均超过该模式感染者总数的50%以上。结论深圳市外来劳务工乙肝5项异常者近半数携带HBV DNA,必须加强对大三阳和模式D组（HBsAg阳性和抗-HBc阳性）感染者以及20~30岁的青年劳务工的HBV DNA的监测,从而对外来劳务工HBV感染者的传染性进行正确的评估,为乙肝的防护和治疗提供依据。

Objective To understand the HBV infection mode and the status among out-labor workers of Shenzhen during 2007-2009 and explore the relationship between HBV infection mode and the positive rate of HBV-DNA,so as to provide scientific basis for establishing prevent and control strategy for hepatitis B.Methods Real-time PCR and ELISA were used to detect the serological markers of HBV DNA and HBV among the 1 226 out-labor workers of Shenzhen during 2007-2009.Results The total positive rate of HBV DNA among the 1 226 out-labor workers of Shenzhen with abnormal HBV serological biomarkers was 48.94%.Seven infection modes were found and mode B（HBsAg＋anti-HBc＋anti-HBe＋）patients accounted for 49.67% of the total HBV infection patients.The positive rate of HBV DNA for mode A and B were 94.11%and 21.67%,respectively.The number of HBV-infected out-labor workers at age 20-30 accounted for more than 50% of the A-G infection modes patients.Conclusion Nearly 50% out-labor workers of Shenzhen with abnormal HBV serological biomarkers are carrying HBV DNA.It is necessary to strengthen HBV DNA surveillance for the out-labor workers with infection mode A and D,including the young out labor workers at age 20-30 so as to determine the infectivity correctly and provide basis for the prevention and treatment of hepatitis B.