摘要
目的观察乙型肝炎病毒(HBV)感染者HBV DNA的含量与T淋巴细胞亚群的相关性,探讨HBV感染者细胞免疫功能的变化。方法收集60例HBV感染者的血清,用荧光定量PCR仪检测其HBV DNA的含量,按HBV DNA的浓度分为HBV DNA阴性组(〈500.00 copy/mL)、HBV DNA低浓度组[(500~1)×105copy/mL]和HBV DNA高浓度组(1×105~1×108copy/mL),另外取20例健康人的血清作对照,同时取上述80例的新鲜外周血,用流式细胞仪检测他们的T淋巴细胞亚群CD3+、CD3+/CD4+、CD3+/CD8+及CD3+CD4+/CD3+、CD8+,对其结果进行分析。结果 HBV DNA阴性组和低浓度组的CD3+CD4+、CD3+CD8+和CD3+CD4+/CD3+CD8+与健康对照组相比,差异无统计学意义(P〉0.05),HBV DNA高浓度组的CD3+CD4+、CD3+CD8+和CD3+CD4+/CD3+CD8+与健康对照组相比,差异有统计学意义(P〈0.05),HBV DNA低浓度组和高浓度组的HBVDNA含量与CD3+CD4+和CD3+CD4+/CD3+CD8+呈现负相关关系,与CD3+CD8+呈现正相关关系,相关系数分别为0.46、0.69和0.52。结论 HBV DNA对细胞免疫产生了影响,HBV DNA含量与T淋巴细胞亚群的分布存在一定的相关性。
Objective To observe the correlation between the content of HBV DNA and T-lymphocyte in HBV infected patients and investigate the variation of cellular immune function in them.Methods We collected the serum of 60 HBV infected patients,and tested the content of HBV DNA with fluorescence quantitative PCR method.According to the concentration of HBV DNA,60 patients were divided into three groups,namely HBV DNA negative group(500.00 copy/mL),low concentration group[(500-1)×105 copy/mL]and high concentration group(1×105~1×108 copy/mL).Then we tested the serum of 20 healthy people as control group,and collected fresh peripheral blood of the 80 samples and tested their T-lymphocyte subsets by flow cytometry,including CD3+,CD3+CD4+,CD3+CD8+ and CD3+CD4+/CD3+CD8+,and then analyzed the results.Results Compared with the control group,the percentage of CD3+,CD3+CD4+ and CD3+CD8+ and the ratio of CD3+CD4+/CD3+CD8+ was not significantly different to the HBV DNA-negative group and low viral concentration group(P0.05),however,the percentage of CD3+,CD3+CD4+ and CD3+CD8+ and the ratio of CD3+CD4+/CD3+CD8+ was significantly different to the high viral concentration group(P0.05).The concentration of HBV DNA in HBV DNA positive groups was negatively correlated with the percentage of CD3+CD4+ as well as the ratio of CD3+CD4+/CD3+CD8+,but positively correlated with the percentage of CD3+CD8+,with the correlation coefficients respectively 0.46,0.69 and 0.52.Conclusion HBV DNA has influence on cellular immunity,and there is a correlation between the content of HBV DNA and the distribution of T-lymphocyte subsets.
出处
《检验医学与临床》
CAS
2011年第8期905-906,908,共3页
Laboratory Medicine and Clinic