检测凝血酶原时间所用试剂最佳预热时间及其参考范围

The optimum preheating time and the reference range of the reagent used in detecting PT

目的用Sysmex CA50自动血液凝固测试仪检测血酶原时间（PT）,确定所用PT试剂（Thromborel（S）的最佳预热范围。方法分别随机抽取门诊患者40例和健康体检者40例,在试剂预热0~14 h内,对血液标本进行检测,对数据进行统计分析比较后确定PT试剂最佳预热时间的参考范围。结果发现在30 min至8 h内检测PT,结果比较稳定。结论在使用Sysmex CA-50检测PT时,应注意PT试剂的老化过程和最佳预热时间才能得到准确、可靠的结果。

Objective To determine the optimum preheating time and the reference range of the reagent in detecting prothombin time（PT）by semi-automated analyzer sysmex CA-50.Methods Forty out-patients and forty healthy people were chosen at random,respectively.We tested the samples by the reagent within 14 hours preheating.Based on statistical analysis of the result,we determined the reference range of the optimum preheating time of the PT reagent.Results It was found that the optimum preheating time of the reagent was between thirty minutes to eight hours and the result was more stable during this range.Conclusion For the purpose of obtaining stable and accurate results,the aging process and optimum preheating time of reagent must be noticed on detecting PT by Sysmex CA-50.