摘要
目的:观察TLR2激活剂Pam3CK对IL-10转染小鼠髓样树突状细胞免疫功能影响。方法:转染IL-10至小鼠髓样树突状细胞(mDC),TLR2配体Pam3CK刺激48小时,利用流式细胞仪检测DC表面标志MHCⅡ、CD80、CD86及FasL等分子的表达;ELISA检测细胞产生IL-6、TNF-α。结果:IL-10抑制mDC表达CD80、CD86、MHCⅡ类分子,降低其分泌IL-6、TNF-α,促进其表达FasL,而TLR2激动剂刺激增加了IL-10转染DC表达MHC-Ⅱ类分子及CD80、CD86,促进其产生IL-6及TNF-α,抑制了FasL表达。结论:TLR2激动剂可逆转IL-10诱发的DC免疫应答低下。
Objective：The effects of TLR2 agonist on the immune function of IL-10 transfected bone-marrow derived dendritic cell（mDC）.Methods：The IL-10 plasmid was transfected to mouse mDC,and TLR2 agonist Pam3CK was added to mDC for 48 hrs.The expression of MHCⅡ,CD80,CD86,FasL in mDC was detected with flow cytometry,and the production of the IL6 and TNF-α was determined by ELISA.Results：IL-10 suppressed the expression of CD80,CD86 and MHCⅡ,decreased the production of IL-6,TNFα,and increased the expression of FasL on mDC.TLR2 agonist Pam3CK increased the expression of MHCⅡ,CD80 and CD86,enhanced the production of IL-6 and TNF-α,but inhibited of FasL expression by the IL-10 transfected mDC.Conclusion：TLR2 activation reverses IL-10 induced low-response of mDC.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2011年第4期300-302,307,共4页
Chinese Journal of Immunology
基金
国家自然基金资助项目(C080701)
