广州地区人类白细胞抗原-Ⅰ类与丙型肝炎病毒-6a型感染相关性的研究

Study on association between hepatitis C-6a and HLA-Ⅰ allele in Guangzhou area

目的：广州地区HLA-Ⅰ类抗原和HCV-6a感染相关性的研究。方法：选取20022008年HCV抗体检测双试剂阳性无偿献血者250名,随机抽取621名广州地区无血缘关系的健康无偿献血者作为对照,用逆转录-套式聚合酶链反应扩增HCVE1和NS5B基因,对E1和NS5B区扩增阳性的PCR产物核苷酸序列测定,将所测定的HCVE1、NS5B进行基因分型。LU-MINEX-SSO方法检测HLA-A,B检测样本HLA-A,B位点。根据公式对HLA型别相关性的相对危险性进行评估。结果：250名无偿献血者中有69个样本的HCVE1和NS5B区分型结果为HCV-6a,占到分析总数的27.6%。A＊33、A＊30与HCV-6a感染相关评估的RR值均大于4,B＊56、B＊54、B＊55、B＊58、B＊76与HCV-6a感染相关评估的RR值均大于4,与HCV-6a感染具有强关联性。结论：广州地区HCV-6a的感染与A＊19和B＊22存在强关联性,对HCV-6a的地域性流行、治疗和预后评价有一定预见作用。

Objective：To study the association between hepatitis C-6a and HLA-Ⅰ allele in Guangzhou area.Methods：250 individuals of HCV antibodies positive and 621 health blood donors in Guangzhou area were enrolled in this study.Nested polymerase chain reaction（PCR） was applied to detect HCV RNA in the sera.The gene sequences in E1 and NS5B area were detected for gene typing of HCV.LUMINEX SSO methods was used to detect the HLA-Ⅰ allele.The relative risk was evaluated in regular formular.Results：There were 69 samples as HCV-6a gene type in the 250 persons.RR value in A＊33,A＊30 was beyond 4,and RR value in B＊56,B＊54,B＊55,B＊58,B＊76 was also more than 4.Conclusion：HLA-A19 and HLA-B22 are important genetic factor for infection of HCV-6a.