RRLC定性检测食用油中10种抗氧化剂的条件研究与应用

Research and application of qualitative detection of 10 antioxidants in edible oil by RRLC

目的建立一种简单、快速、准确测定食用油中多种抗氧化剂的检测方法。方法采用高分离度快速液相色谱仪(RRLC)对食用油中10种常用的抗氧化剂包括没食子酸丙酯(PG)、没食子酸辛酯(OG)、2,4,5-三羟基苯丁酮(THBP)、叔丁基对苯二酚(TBHQ)、叔丁基对羟基茴香醚(BHA)、2,6-二叔丁基对甲酚(BHT)、没食子酸十二酯(DG)、正二氢愈创酸(NDGA)、2,6-二叔丁基-4-羟甲基苯酚(HMBP)和没食子酸异戊酯(IAG)进行定性检测,并对使用RRLC定性检测食用油中的抗氧化剂的分离条件进行摸索。结果以1.5%乙酸的甲醇溶液和1.5%乙酸的水溶液作为流动相进行梯度洗脱,可以有效地分离并用于食用油中抗氧化剂的检测。结论此方法简便、快速,实用性强,在保证实验准确、灵敏的前提下,大大缩短了分析流程。

Objective To establish an easy,fast and accurate method of measuring different antioxidants in edible oil.Methods To study 10 common antioxidants including PG,OG,THBP,TBHQ,BHA,BHT,DG,NDGA,HMBP,and IAG in edible oil by RRLC,select the separate conditions of qualitative detection of 10 antioxidants in edible oil by RRLC.Results Using 1.5% acetic acid in methanol and 1.5% acetic acid in water as mobile phase to gradient can separate 10 antioxidants in edible oil effectively.Conclusions This method is easy,fast and practical,in the premise of ensuring accuracy and sensitivity,it can shorten the analysis process as well.