氟哌啶醇对豚鼠心室肌细胞I_K的影响

Effects of haloperidol on potassium current in isolatated ventricular myocyte of guinea pig

目的观察氟哌啶醇对豚鼠单个心室肌细胞延迟整流钾电流(IK)通道的作用,探讨氟哌啶醇致心律失常的离子基础。方法应用全细胞膜片钳技术记录氟哌啶醇对豚鼠单个心室肌细胞IK的影响。结果在获得单个细胞后,分别给予0.3、1.0、3.0μmol/L的氟哌啶醇。延迟整流钾电流在试验电压+70 mV时,从给药前的(16.660 4±1.571 7)分别减少至(5.025 5±1.937 2)、(9.857 2±3.900 2)、(7.179 4±1.816 0)pA/pF(n=7,P<0.01)。结论氟哌啶醇可抑制IK,使动作电位时程延长而引发心律失常,其致心律失常作用可能与其对钾通道的作用有关。

Objevtive To investigate the effects of haloperidol on delayed rectified potassium current in guinea pig ventricular myocyte.Methods Whole-cell patch-clamp was used to record delayed rectified potassium current（Ik）.Results In single ventricular myocytes of guinea pig,haloperidol at concentration of 0.3,1,3 μmol/L respectively decreased Ik from（16.660 4±1.571 7） pA/pF to（5.025 5±1.937 2） pA/pF,（9.857 2±3.900 2） pA/pF and（7.179 4±1.816 0）pA/pF.Conclusion The induced arrythmic effects of haloperidol may be related to delayed rectified potassium current.