染料脱色酶特性研究

STUDY ON CHARACTERIZATION OF DECOLORIZING ENZYME

从脱色优势菌ＮＤ１ 提取脱色酶，对酶特性进行研究．结果表明酶的最适作用条件为ｐＨ ７，３７℃，无氧环境，反应需ＮＡＤＨ 的参与；纯化结果表明经ＤＥＡＥ柱层析及Ｓｅｐｈａｄｅｘ Ｇ－１００ 凝胶层析后脱色速率分别提高１０ 倍和５０倍，Ｓｅｐｈａｄｅｘ Ｇ－１００ 凝胶层析中２～４ 管蛋白具有偶氮和蒽醌染料脱色酶活性，６～１２ 管具有蒽醌染料脱色酶活性．

In this paper, decolorizing enzyme of superior strain ND1 was extracted and purified, and the characterization of enzyme was studied. The result showed that the optimum condition was pH7.0, 37℃,anaerobic, NADH added. By DEAE and Sephadex G 100 chromatogram purified, the decoloriziing rate of the enzyme was improved 10 and 50 times respectively, and in Sephadex G 100 chromatogram, the protein in tube 2～4 was in charge of the decolorization of zao and anthraquinone dyes, tube 6～12 was in charg of decolorizing enzyme activity of anthraquinone dye.