纳米氧化锌对绿豆芽生长的影响

Effects of Nano-ZnO Particles on the Growth of Green Bean Sprouts

深入研究不同类型的纳米颗粒对不同植物物种具有的独特效应,可提高有关纳米物质对植物安全与风险的认知水平。为了对纳米物质生物效应的深入评估提供科学依据,测定了室温（25℃）浸种培养后绿豆芽的叶绿素、过氧化物酶活性、根长、茎长、鲜重、干重、蛋白质等生理指标及其可食部分的锌含量。结果表明,nano-ZnO和Zn2＋在较高浓度（50~1000mg.L-1）时均可抑制植物的生长,呈现出一定的植物毒性;相同浓度时,nano-ZnO较Zn2＋更有利于绿豆芽的生长和其可食部分锌的富集。最有利于绿豆芽生长和锌富集的nano-ZnO浓度为50mg.L-1,而Zn2＋浓度则为20mg.L-1。在促进生长的浓度范围内（0~50mg.L-1）,绿豆芽可食部分锌含量均小于国家食品锌标准含量值（20mg.kg-1）。可见,nano-ZnO可尝试应用于绿豆芽的农业生产活动,但在此之前须进一步开展其存在的环境、生态与人体健康风险研究。

Different types of nanoparticles and plant species need to be examined to clarify nanoparticles uptake by plant and the subsequent effects,which helps to understand deeply the risk of nanomaterials.In this study,some physiological indicators of green bend sprouts such as the content of chlorophyll,peroxidase activity,protein content,root length,grass height,fresh weight and dry weight as well as the content of zinc in its edible parts were determined after cultured in nano-ZnO solution in room temperature（25 ℃）,which will provide insights into the bioeffects of nanomaterials.The results showed that the phytotoxicity of both nano-ZnO and Zn2＋ with higher concentrations（50～1 000 mg·L-1）occurred,beyond which the growth of green bean sprouts were inhibited.In the same concentrations,the growth of green sprouts and the znic enrichment in its edible parts were improved more easily by nano-ZnO than by Zn2＋.Additionally,50 mg·L-1 of nano-ZnO was the best treatment concentration for the growth of green sprouts and the znic enrichment in its edible parts,while that of Zn2＋ was 20 mg·L-1.At the range of 0～50 mg·L-1,the znic contents of edible parts of green bean sprouts were less than 20 mgZn kg-1（National znic standard of food in Chinese）.Therefore,nano-ZnO could be applied tentatively into agricultural practices of green bean sprouts.However,it is necessary to understand further the human health,ecological and environmental risks of nano-ZnO,on which were seriously concerned so far.