苯丙烯酸胁迫对黄瓜幼苗生长和抗氧化代谢特性的影响

Effects of Cinnamic Acid Stress on the Growth and Antioxidant Metabolism of Seedlings in Cucumber(Cucumis sativus L.)

采用营养钵土培的方法,研究了外源苯丙烯酸对津美1号、绿衣天使、C90和翠龙等不同黄瓜品种幼苗生长和叶片抗氧化酶活性及活性氧代谢的影响。结果表明,外源苯丙烯酸胁迫对黄瓜幼苗生长具有很强的抑制作用,并随着胁迫浓度的增大受抑制的程度加强。在苯丙烯酸胁迫下,黄瓜叶片的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性表现为低浓度升高,高浓度下降的趋势;超氧阴离子(O2-.)产生速率、过氧化氢(H2O2)含量、膜脂过氧化产物丙二醛(MDA)含量及渗透调节物质脯氨酸(Pro)含量、可溶性糖含量随着苯丙烯酸浓度的升高而显著增加。不同黄瓜品种对苯丙烯酸胁迫的响应存在着品种间差异。

The effects of exogenous cinnamic acid stress on seedling growth,antioxidant enzyme activities and reactive oxygen species metabolism of different varieties of cucumber,such as Jinmei No.1,Lvyitianshi,C90 and Cuilong,were studied by planting in the plastic pot filled with nutrient soil.The results showed that,exogenous cinnamic acid had a strong inhibitory effect on seedlings growth of cucumber,this inhibitory effect was much stronger with the increasing concentration of cinnamic acid.The activities of antioxidant enzymes including,SOD,POD,CAT and APX,showed upward trend when the concentration of cinnamic acid was low（50～100 μmol·L-1）,but the trend was downward at high dose（200～400 μmol·L-1）.The generation rate of O-2·,the contents of H2O2,MDA,Pro and soluble sugar were significantly increased as the raising amount of cinnamic acid added to the soil.Above mentioned parameters were different among varieties of cucumber under cinnamic acid stress.