胃转流术治疗非肥胖型2型糖尿病大鼠的长期疗效观察

Long-term efficacy of gastric bypass on non-obese type 2 diabetes mellitus in Goto-Kakizaki rat

目的探讨Roux-en-Y式胃转流术治疗非肥胖型2型糖尿病大鼠的安全性及稳定性。方法雄性Goto-Kak-izaki(GK)大鼠及Wistar大鼠两组,每组12只,均行Roux-en-Y式胃转流术。检测术前及术后1周、1个月、3个月、6个月、12个月、18个月、24个月体质量,空腹血糖及糖化血红蛋白水平。结果术后各组大鼠体质量未见减少(P>0.05),随年龄增长逐渐增加。与术前相比较,Wistar组空腹血糖、糖化血红蛋白术后24个月内未见明显变化(P>0.05);GK大鼠组术后空腹血糖显著降低(P<0.01),糖化血红蛋白术后3个月开始降低(P<0.01),并长时间保持稳定状态,术后两组大鼠各死亡1只,死亡率低。结论 Roux-en-Y式胃转流术可长期有效控制非肥胖型2型糖尿病大鼠血糖水平,对正常体重的Wistar大鼠血糖无影响;手术并发症少、安全、稳定。

Objective To investigate the safety and feasibility of Roux-en-Y gastric bypass in non-obese type 2 diabetes rats.Methods Goto-Kakizaki rats and Wistar rats underwent gastric bypass with anastomosis of Roux-en-Y,divided into two groups （n=12）.Rats were observed for 24 months after surgery.Fasting blood glucose、glycosylated hemoglobin A1c and body weight were tested at 0,1 weeks,1,3,6,12,18,24 months.Results After surgery the body weight kept the similar level during the entire follow-up period （P〉0.05） and progressively increased with the age increased.Compared with preoperation,fasting blood glucose levels and glycosylated hemoglobin A1c levels were similar during the entire follow-up period in Wistar group.In GK group,the fasting blood glucose levels were significantly decreased as early as 1 week after surgery and then kept a similar level during the entire follow-up period （P〈0.01）,the glycosylated hemoglobin A1c level were conspicuously decreased in 3 month after the surgery and then kept a similar level during the entire follow-up period （P〈0.01）.Two rats were died after surgery and the mortality rate was low.Conclusions Our data show that Roux-en-Y gastric bypass in non-obese type 2 diabetes model were effective in terms of glucose control,but ineffective in Wistar rats whose weigh were normal.The operation is safety and feasibility.