促甲状腺激素β基因剪接变体在BALB C小鼠不同组织中的表达

A Novel Thyroid Stimulating Hormone β-Subunit Splice Variant in Bone Marrow and Other Tissues in BALB C Mice

目的：研究促甲状腺激素（TSH）β基因剪接变体在BALBC小鼠垂体、甲状腺、骨髓、脾、外周血和淋巴结中的表达情况。方法：选取雄性6~8周龄BALBC小鼠6只,提取小鼠垂体、甲状腺、骨髓、脾、外周血和淋巴结的总RNA,用DNA酶处理后,应用逆转录聚合酶链反应（RT-PCR）方法检测TSHβ剪接变体的表达情况。结果：垂体、甲状腺、骨髓、脾、外周血和淋巴结中均可见TSHβ剪接变体的特异性表达,且在垂体和外周血中的表达量较高。测序证实该剪接体含有部分第4内含子和整个第5外显子的编码区,没有第4外显子。结论：TSHβ剪接变体在BALBC鼠的垂体、甲状腺、骨髓、脾、外周血和淋巴结中均有表达。

Objective：To determine whether the novel thyroid stimulating hormone β-subunit （TSHβ） splice variant was expressed in pituitary, thyroid tissues,bone marrow（BM）, spleen, peripheral blood and lymph nodes of BALB C mice. Methods：Six male BALB C mice （6-8 week-old） were selected in this study. Total RNA was isolated respectively from pituitary, thyroid tissues, BM, spleen, peripheral blood leukocytes （PBL） and lymph nodes of BALB C mice. The samples were treated with an RNase-free DNase. Reverse transcriptase polymerase chain reaction （RT-PCR） was performed to detect the expression of TSHβ splice variant in these tissues. Results：The TSHβ splice variant was expressed in pituitary, thyroid tissues, BM, spleen, PBL and lymph nodes in BALB C mice. The level of the TSHβ splice variant expression was higher in pituitary and PBL than that of other tissues.？The sequence analysis confirmed that it consisted of a portion of intron 4 and all of the coding regions of exon 5, but none of exon 4 codon. Conclusion：The TSHβ splice variant is expressed in pituitary, thyroid tissues, BM, spleen, PBL and lymph nodes in BALB C mice.