芳香化酶抑制剂对大鼠子宫内膜异位灶中生存素及Caspase-3的影响

Effects of Aromatase Inhibitor on Expressions of Survivin and Caspase-3 in the Ectopic Focus of Rat Model with Endometriosis

目的:探讨芳香化酶抑制剂对子宫内膜异位灶中生存素(Survivin)及半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)表达的影响。方法:采用自体子宫内膜移植法建立大鼠子宫内膜异位症(EMs)模型,造模3周后开腹观察、测量异位灶形态及大小,计算体积,将成模大鼠随机分为来曲唑治疗组(A组)及模型对照组(B组),每组20只。给药4周后处死,观察并测量2组异位灶形态、大小,及2组异位内膜及正常内膜组(C组)的内膜组织学形态,并采用免疫组化法检测Survivin及Caspase-3表达情况。结果:给药4周后,A组异位灶较给药前减小(P<0.05);Survivin及Caspase-3均在内膜腺上皮细胞表达,定位于细胞浆。Survivin在A组及C组的阳性率均低于B组(均P<0.01),Caspase-3在A组及C组的阳性率均高于B组(均P<0.01),而Survivin及Caspase-3在A组与C组阳性率差别无统计学意义(P>0.05)。异位灶中Survivin与Caspase-3的表达呈负相关(rs=-0.538,P<0.05)。结论:芳香化酶抑制剂可抑制大鼠EMs病灶的生长,并抑制异位灶中Survivin的表达,增加Caspase-3的表达。

Objective： To explore the effect of aromatase inhibitor on the expressions of Survivin and Caspase-3 in ectopic focus of rat model. Methods： The rat model of endometriosis（EMs）was made by autologous transplantation of endometrium. The shape and size of the ectopic foci in abdominal cavity were observed and measured after 3 weeks. Rats were then randomly divided into two groups （ twenty in each）, letrozole group（A group）and model group as the control （B group）. After 4 weeks of administration, the endometrial histological changes were observed in A group, B group and the normal endometrium group （C group）. The expression levels of Survivin and Caspase-3 were assessed by immunohistochemical staining in ectopic endometrium and normal endometrium. Results： The rat model of EMs was successfully established. The size of ectopic focus was significantly reduced after 4 weeks of treatment compared with that of before treatment in A group（P〈0.05）. Both Survivin and Caspase-3 were expressed in endometrial glandular epithelial cells, locating in cytoplasm. Results showed that the positive rate of Survivin was lower in A group and C group than that of B group （P〈0.01）. The positive rate of Caspase-3 was higher in A group and C group than that of B group （P〈0.01）, but there was no significant difference in positive rates of Survivin and Caspase-3 between A group and C group （P〉0.05）. The expression of Survivin was negatively correlated with that of Caspase-3（rs =-0.538,P〈0.05）. Conclusion： The aromatase inhibitor can inhibit the growth of ectopic focus and Survivin expression, and increase the expression of Caspase-3 in rat ectopic endometrial lesions. The results may offer theoretical foundation for clinical application of letrozole in treatment of EMs.