职业性慢性铅中毒患者CD8低表达T淋巴细胞数量观察

Abnormal increase in CD8lowT lymphocyte in patients with occupational chronic lead poisoning

目的通过分析外周血T淋巴细胞CD8分子表达水平，了解职业性慢性铅中毒患者异常的免疫功能状态。方法对23例职业性慢性铅中毒患者（铅中毒组）及20例健康非职业铅接触成人（对照组）采用流式细胞技术检测外周血T淋巴细胞CD8分子表达水平，统计CD8低表达（CD8h）细胞群和正常表达（CD8norrnal）细胞群的数量。结果与对照组（8．21％±3．02％）比较，铅中毒组CD8low细胞群相对百分比（12．98％±5．62％）明显增加，差异有统计学意义（P〈0．05）；两组CD8norrnal细胞群数量则无明显变化。结论职业性慢性铅中毒患者外周血CD8＋T淋巴细胞虽然总数没有异常，但CD8分子表达出现了弱化。该现象可能是铅致免疫功能损伤重要表现之一，CD8low细胞群有望成为研究铅免疫毒性新的线索。

Objective To analyze the changes in CD8low T lymphocyte subsets in patients with occupational chronic lead poisoning. Methods Flow cytometric analysis was used to count the numbers of CD8 ± ceils. 23 patients with occupational chronic lead poisoning and 20 controls were examined. Results Compared with control group （8.21%±3.02%）, the CD8low T lymphocyte （12.98%±5.62%） were significantly increased in patients with occupational chronic lead poisoning. Conclusion Although the ratio of CD ＋T lymphocyte is normal, the CD8 level is significantly decreased. The increase of CD81~ T lymphocyte may be an important phenomenon of immuno-injury induced by lead. CD8low T lymphocyte could be an new direction for research of lead immnnn-toxicity.