急性百草枯中毒大鼠肾损害时血清中炎性因子的变化

Clinical study of dynamic change of inflammatory factors in serum of acute paraquat rats

目的观察急性百草枯（PQ）中毒大鼠血清中炎性因子的变化，探讨炎性因子在急性PQ中毒肾损伤中的作用。方法100只SD雄性大鼠随即分成正常对照组（20只，生理盐水腹腔注射）和PQ染毒组（80只，腹腔注射PQ25mg／kg）。PQ组于染毒后6h及1、3、7d处死，正常对照组于处理后1d处死，检测血清中肿瘤坏死因子-α（TNF—α）、白细胞介素-2（IL-2）、IL-6含量，并观察肾组织的病理改变。结果PQ染毒组大鼠肾组织早期病理表现为充血水肿，少量炎性细胞浸润，染毒组在不同时间点病理损伤的严重程度无明显差别，死亡大鼠。肾组织细胞核固缩、细胞结构溶解等病理改变。与正常对照组相比，PQ染毒组大鼠6h和1、3、7d时IL-2水平分别为（2．16±0．65）、（2．95±1．02）、（3．05±1．12）、（2．21±0．62）0,g／L，IL-6分别为（62．5±8．6）、（85．6±13．5）、（90．3±15．6）、（65．3±9．1）ng／m]，TNF-α分别为（1．95±0．53）、（2．86±0．92）、（3．15±1．02）、（2．06±0．71）μg/L，与对照组[IL-2：（1．71±0．45）μg／L，IL-6（38．2±7．5）ng／ml，TNF-α（1．22±0．32）μg／L]比较，均明显升高，差异均有统计学意义（火O．01）。结论急性PQ中毒大鼠血清中TNF-α、IL-2、IL-6水平明显升高，均参与PQ急性中毒肾损伤的致病过程。

Objective To investigate the change of inflammatory factor in lung tissue of acute paraquat （PQ） poisoned rats. Methods hundred SD rats were randomizedly divided into two groups：the normal control group （NC group,n=10）and the PQ group （n=80）.The 1 ml saline was administered once in normal control group.The PQ group was administered with 25 mg/kg 1% PQ by intraperitoneal injection to establish the model of PQ induced renal injury.At six hours,at the first,the third and the seventh day the PQ group were sacrificed, while at the first day the normal control group was sacrificed.The level of normal tumor necrosis faetor-α （TNF-α）, interleukin -2 （IL-2）, IL-6 in serum of rats were detected. Meanwhile, pathological changes of the renal were examined under optical microscope. Results Histopathological findings of an earlier, a large number of patients edema clearly inflammatory cell infiltration. Compared with the control group, PQ exposure of serum TNF-α, IL-2, IL-6, the level at each time point were elevated. PQ treated group 6 h and 1, 3, 7 d when the IL-2 levels were （2.16±0.65）, （2.95±1.02）, （3.05±1.12）, （2.21±0.62）μg/L, IL-6 were （62.5± 8.6）, （85.6±13.5）, （90.3±15.6）, （65.3±9.1）ng/ml, TNF-α were （1.95±0.53）, （2.86±0.92）, （3.15±1.02）, （2.06 ±0.71 ）p,g/L, compared with the control group, are significantly higher, the differences were statistically significant （P〈0.01）. Conclusion acute PQ poisoning serum TNF-α, IL-2, IL-6 levels were significantly increased both early and late inflammatory factors involved in PQ poisoning the pathogenesis of renal injury.