利用转座标签mTn-lacZ/leu2插入突变发酵性丝孢酵母2.1368-Leu^-筛选高效产油突变株

Screening of high lipid production Trichosporon fermentans mutants by transposon tagging mTn-lacZ/leu2 insertion

为提高微生物油脂产率,降低其生产成本,以转座标签mTn-lacZ/leu2插入突变发酵性丝孢酵母2.1368-Leu-筛选高效产油突变株。利用LacZ显色反应、脂肪酸合成酶抑制剂Cerulenin和磷酸香草醛反应,最终在玉米秸秆糖化液中筛选出一株高效产油突变株2.1368-Leu--7。结果表明其油脂含量为38.30%,比对照的29.33%高了8.97%,而其产油率为8.35%,比对照的6.92%提高了20.63%;在玉米秸秆糖化液中的糖利用率为77%,每100 g玉米秸秆可转化油脂8.32 g。可为未来生物柴油产业提供了廉价原料。

To improve microbial lipid production,we inserted mTn-lacZ/leu2 into Trichosporon fermentans 2.1368-Leu- to obtain high lipid production mutants.By observing the LacZ chromogenic change,the positive reaction between Cerulenin（inhibitor of fatty acid synthase） and phosphate vanillin,a higher lipid-producing mutant 2.1368-Leu--7 grown on corn-stalk hydrolysate was obtained.The lipid content of this mutant reached 38.30%（8.97% higher than that of the control） and the lipid production rate was 8.35%（20.63% higher than that of the control）.The rate of sugar utilization was 77%,meaning that 100 g corn-stalk could be converted to 8.32 g lipid.The study provided an effective method for microbial lipid production by using cheap raw materials for biodiesel.