TSP1基因G1678A和A2210G基因多态性与贲门腺癌发病风险的关联

Correlation of TSP1 G1678A and A2210G Polymorphisms to Risk of Gastric Cardia Adenocarcinoma

目的探讨凝血酶敏感蛋白-1（TSP1）基因第10外显子G1678A和第13外显子A2210G单核苷酸多态性（SNP）与中国北方人群贲门腺癌（GCA）遗传易感性的关系。方法采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析方法检测145名GCA患者和198名健康对照的TSP1G1678A和A2210G多态性分布情况。结果 TSP1G1678A多态性位点的基因型及等位基因型频率在贲门腺癌患者组和对照组之间,其总体分布差异均无统计学意义（P〉0.05）。根据吸烟状况和上消化道肿瘤家族史分层分析发现,与GG和GA基因型相比,携带AA基因型可能增加非吸烟个体贲门腺癌的发病风险（经性别、年龄和上消化道肿瘤家族史校正后的OR为1.79,95%CI为1.46~2.11）。TSP1A2210G位点G等位基因频率在对照组人群中〈1%,它可能不是我国北方人群中的常见多态。结论 TSP1基因第10外显子AA基因型可能是影响我国北方人群中非吸烟个体贲门腺癌发病风险的因素之一。

Objective To investigate the possible association of TSP1 exon10 G1678A and exon13 A2210G polymorphisms with susceptibility to gastric cardia adenocarcinoma（GCA） in a population of North China.Methods The TSP1 G1678A and A2210G single nucleotide polymorphisms（SNPs） were genotyped by polymerase-chain reaction-restriction fragment length polymorphism（PCR-RFLP） analysis in 145 GCA patients and 198 healthy controls.Results The overall genotype and allelotype distributions of TSP1 G1678A in GCA patients were not significantly different from those in healthy controls（P0.05）.When stratified for smoking status and upper gastrointestinal cancers（UGIC） family history,compared with GG and GA genotype,AA genotype significantly increased the risk of developing GCA in non-smoker group [age and gender and UGIC family history adjusted odds ratio（OR）= 1.79,95%CI= 1.46~2.11].The frequency of G allelotype was less than 1%,which indicated that TSP1 A2210G was not a common polymorphism in north China.Conclusion AA genotype of TSP1 G1678A may be one of the factors that affect the risk of developing GCA in non-smoking population in north China.