木脂素分解产物enterolactone和enterodiol对成骨细胞样细胞MG63的双相作用

Biphasic effect of lignanoid decomposition products:enterolactone and enterodiol on osteoblast-like MG63 cells

目的:观察enterolactone和enterodiol对成骨细胞样细胞MG63的作用。方法:用MTT法检测观察enterolactone和enterodiol对MG63细胞生长的影响,用碱性磷酸酶活性检测观察enterolactone和enterodiol对MG63细胞活性的影响,用逆转录聚合酶链反应(RT-PCR)观察enterolactone和enterodiol对MG63细胞碱性磷酸酶、骨钙素、Ⅰ型胶原基因表达水平的影响。结果:Enterolactone和enterodiol在0.01 mg/mL浓度时能增加MG63细胞的活力。随着浓度的增加,细胞活力逐渐减弱。Enterolactone和en-terodiol对MG63细胞碱性磷酸酶活性的影响与对MG63细胞生长的影响基本一致,也是在低浓度时增加其活性,在高浓度时抑制其活性。En-terolactone和enterodiol都能增加MG63细胞碱性磷酸酶、骨钙素和Ⅰ型胶原的基因转录。结论:Enterolactone和enterodiol在低浓度时能增进成骨细胞的活性,在高浓度时抑制其活性,对成骨细胞有双相调节作用。

AIM ： To observe the effects of enterodiol and enterolactone on osteoblastic MG63 cells. METHODS ： The effects of enterodiol and enterolactone on the proliferation of MG63 cells were observed by MTT.The effects of enterodiol and enterolactone on the activity of MG63 cells were tested by alkaline phosphatase（ALP）test.The effects of enterodiol and enterolactone on transcriptional level of a ALP,osteocalcin,and collagenⅠof MG63 cells were measured by RT-PCR. RESULTS ：The cell viability of MG63 cells significantly was increased when treated with 0.01 mg/mL enterolactone or enterodiol.The viability of MG63 cells was gradually weakened with the increase of concentration.Similar to their effects on the cell viability of MG63 cells,enterolactone or enterodiol increased the ALP activity of MG63 cells at low concentration and decreased the ALP activity at high concentration.Enterolactone or enterodiol can increase the transcriptional level of ALP,collagenⅠ,and osteocalcin in MG63 cells. CONCLUSION ： The osteoblast cells viability is increased at low concentration treated with enterolactone and enterodiol,but that is decreased at high concentration.Enterolactone and enterodiol have biphasic effect on MG63 cells.