2006-2008年深圳市食源性病例和外环境的副溶血性弧菌分离株分型特征比较

Characterization of Vibrio parahaemolyticus obtained from environment and cases of foodborne disease in Shenzhen,during 2006-2008

【目的】比较深圳市食源性病例和外环境中分离的副溶血性弧菌在血清分布、毒力基因携带情况和分子分型方面的特征。【方法】血清凝集法检测菌株血清型,多重PCR检测毒力基因tdh和trh基因携带情况,脉冲场凝胶电泳(PFGE)分析基因分型特征。【结果】98株食源性病例分离株的主要血清型为O3:K6(40.8%)、O1:KUT(7.1%)、O4:K8(7.1%),35株环境分离株中未发现O3:K6。83.7%(82/98)的食源性病例分离株携带tdh基因、不携带trh基因,88.6%(31/35)的环境分离株不携带tdh基因和trh基因。PFGE显示,最主要的克隆群是P1,包括全部的O3:K6(29株),O4:K68(4株),O11:K36(3株),O1:K25(2株),都是食源性病例分离株,没有环境分离株。环境分离株中未见明显的克隆群。【结论】深圳市食源性病例中分离的副溶血性弧菌多数具有溶血性、携带tdh基因,不携带trh基因,基因分型显示具有优势菌群,主要血清群为O3:K6;环境分离株血清群散在分布,多数不具溶血性,不携带tdh和trh基因,基因分布成多态性,没有发现优势菌群。

[Objective]To analyze the serological and genetic divergence in the Vibrio parahaemolyticus from environment and cases of foodborne disease,and to compare these two groups in terms of virulence and other biological traits.[Methods]Serotyping,multi-PCR,and pulse-field gel electrophoresis（PFGE） were carried out.[Results]The main serotypes of cases isolates were O3： K6（40.8%）,O1： KUT（7.1%）,O4： K8（7.1%）,and the main serotypes of environmental isolates were O3： KUT（14.2%）,O1： KUT（11.4%） and O2： K3（11.4%）.No O3： K6 strain was isolated from environment.Most cases isolates were tdh positive and trh negative,which account for 83.7%,while most environmental isolates were tdh negative and trh negative,which account for 88.6%.PFGE indicated that Clone P1 was the dominant clone cluster,including serotype O3：K6（29 isolates）,O4：K68（4 isolates）,O11：K36（3 isolates）,O1： K25（2 isolates）,and these strains were all obtained from cases of foodborne disease.No dominant clone cluster was found in environmental isolates.[Conclusions]V.parahaemolyticus from cases of foodborne disease in Shenzhen mostly were hemolytic,tdh positive and trh negative,there were dominant PFGE type in cases isolates,the main serotype was O3：K6,while environmental isolates mostly were non-hemolytic,tdh negative and trh negative,no dominant PFGE type was found.