云南程海湖酵母菌多样性及应用

Identification of yeasts isolated from Chenghai Lake,a plateau lake in Yunnan province

【目的】针对云南丽江永胜县境内程海湖环境的特殊性,研究高原湖泊环境中酵母菌的多样性,初步探索程海湖环境中酵母菌的利用价值。【方法】对程海湖的湖水和其周边土壤样品中的酵母菌进行分离;应用26S rDNA的D1/D2区域序列分析,并结合形态及生理生化指标对分离获得的酵母菌进行鉴定;采用筛选培养基对已鉴定酵母菌进行产酶定性实验,分析高原湖泊中酵母菌的多样性及可应用性。【结果】分离得到酵母菌64株,对其中63株进行鉴定,归属于9个属22个种(包括4个疑似新种或新变种);地霉属Geotrichum和隐球酵母属Cryptococcus是2种环境中的共有属;在产酶活性筛选中发现有9株产胞外酶活性的菌株,其中YM24373既产蛋白酶又可产淀粉酶。【结论】研究结果显示程海湖中酵母菌组成具有较为丰富的多样性,其应用价值值得进一步研究。

[Objective]The aims of this study were to investigate yeast diversity in the plateau lake and to explore the value of these yeasts in such ecological environment.[Methods]Yeasts were isolated from 9 water and 4 soil samples collected in Chenghai lake in Yunnan province.The isolates were identified by using large-subunit（26S） rDNA gene D1 / D2 domain sequence analysis and traditional methods.The ability of the yeast strains to produced various enzymes was tested.[Results]In total 64 yeast strains were isolated,these strains were identified as 22 species in 8 genera,including 4 suspected new species or variety.Genera Cryptococcus and Geotrichum were shared in the water and soil samples.Nine strains with secreted enzyme activities were selected.One of them could produce both protease and amylase.[Conclusion]The preliminary result showed the biodiversity of yeasts in Chenghai lake and the application potential of the yeasts isolated.