摘要
为了优化山羊核移植胚胎体外培养体系,提高核移植效率,本研究检测了山羊体细胞核移植(SCNT)胚胎在序贯培养液G1/G2中的发育率和囊胚细胞凋亡,以及核移植胚胎移植后的妊娠率,以传统mSOF-FBS培养液作为对照组,评估序贯培养液G1/G2支持山羊核移植胚胎的发育能力。结果显示,与对照组相比,G1/G2组的囊胚发育率差异不显著((27.7±3.1)%vs(25.3±1.0)%,P>0.05),囊胚细胞数和囊胚细胞凋亡率显著降低(分别为(93.2±4.5)vs(109.1±6.2)和(4.9±0.2)%vs(11.3±0.1)%,P<0.05),但移植后的妊娠率显著增高(21.4%vs 8.0%,P<0.05)。结果表明,与传统的培养液mSOF-FBS相比,序贯培养液G1/G2能更好地支持山羊核移植胚胎的发育。
The aims of this study were to investigate the effects of sequential media G1/G2 and modified synthetic oviductal fluid（mSOF） culture systems on developmental competence,cell apoptosis and the pregnant rate of goat somatic cell nuclear transfer（SCNT） embryos.Goat NT embryos were cultured as following：（1） 72 h in G1 then 120 h in G2（group G1G2）,both the culture media supplemented with 0.8% BSA（bovine serum albumin）;（2） 72 h in mSOF supplemented with 0.8% BSA then in mSOF supplemented with 10% FBS（fetal bovine serum） for 120 h（group mSOF-FBS）.The results indicated that there was no significant difference between the sequential media G1/G2 and mSOF-FBS in blastocyst rates（（27.7±3.1）% vs（25.3±1.0）%,P0.05）.Both of total cell number of blastocysts and apoptotic nuclei in blastocyst from G1/G2 were significantly lower than that in mSOF-FBS（（93.2±4.5） vs（109.1±6.2） and（4.9±0.2）% vs（11.3±0.1）%,respectively,P0.05）.However,the pregnant rates on Day 30 in group G1/G2 were higher than those in group mSOF-FBS（21.4% vs 8.0%,P0.05）.In conclusion,compared to mSOF-FBS,the sequential media G1/G2 could better support the development of goat somatic cell nuclear transfer embryos.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2011年第3期442-447,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家转基因生物新品种培育科技重大专项(2008ZX08007-004)资助
