隐孢子虫感染小鼠MHC-Ⅱ类抗原的表达

Expression of major histocompatibility complex-Ⅱ on mouse cryptosporidiosis

目的探讨中药补骨脂、双氢青蒿素二者配伍对小鼠隐孢子虫病的疗效。研究该合剂对MHC-Ⅱ类抗原表达的调节作用。方法将60只昆明小鼠随机分成5组:A:正常组;B:阳性对照组1;C:1w治疗组;D:阳性对照组2;E:2w治疗组。B、C、D、E四组小鼠经腹股沟皮下注射地塞米松磷酸钠注射液,建立隐孢子虫感染模型。经1w和2w药物治疗后,计数各组小鼠粪便卵囊数量并观察回肠组织中MHC-Ⅱ类抗原的表达。结果所有药物治疗组,粪便卵囊量与阳性对照组相比均明显减少(P(0.05)。在经药物治疗1w组,B组MHC-Ⅱ类抗原的表达显著高于A组(P(0.05),C组与B组无显著性差异。治疗2w后,E组MHC-Ⅱ类抗原的表达与正常对照组A组无差别(P>0.05),D组MHC-Ⅱ类抗原的表达显著低于A组(P<0.05)。结论 MHC-Ⅱ类抗原在隐孢子虫感染小鼠中表达升高,随着虫体感染加重,MHC-Ⅱ类抗原表达受抑;补骨脂、双氢青蒿素合剂可通过调节MHC-Ⅱ类抗原的表达,进而调节宿主免疫应答反应的能力,达到抑杀虫体,修复病损的目的。

To observe the treating function of Fructus Psoraleae and dihydroartemisinin on mouse infected with Cryptosporidium parvum and to research the regulating function of major histocompatibility complex-Ⅱ,60 mice were randomly divided into five groups： normal group A;positive control group-Ⅰ B;one-week treatment group C;positive control group-Ⅱ D;two-week treatment group E.Mice in group B,C,D,and E were injected subcutaneously with dexamethasone.When the mice were infected with C.parvum,they were treated with the medicine respectively.The intestine tissue was observed via HE.The expression of MHC-Ⅱ were observed by immunohistochemistry.The damaged intestine got improved and repaired,and a significant decrease of the amount of C.parvum oocysts in feces was shown in the treated groups（C,E） compared with positive control groups（B,D）（P0.05）.In 1-week treatment group,the MHC-Ⅱ expression of group B was higher than that of normal group A.There was no difference between the expression of MHC-Ⅱ in group B and that in group C;in 2-week treatment group,there was no difference between the expression of MHC-Ⅱ in group E and that in group A.The MHC-Ⅱ expression of group D was lower than that of group A and B（P0.05）.It＇s suggested that fructus Psoraleae and dihydroartemisinin are effective to treat mouse cryptosporidiosis.The expression of MHC-Ⅱ might be regulated by the mixture.