碱性成纤维细胞生长因子对体外培养人牙髓细胞增殖和迁移的影响

Effect of bFGF on the proliferation and migration of human dental pulp cells in vitro

目的研究碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)对体外培养人牙髓细胞增殖、迁移活性的影响。方法以常规组织块培养法体外获得人牙髓细胞,实验组分别加入终浓度为1.0、5.0、10.0和50.0ng/ml的bFGF,对照组仅加入等量的空白培养基,采用CCK-8法分别测定450nm下各组光吸收度A值,研究bFGF对牙髓细胞增殖活性的影响;应用Transwell小室培养法,研究bFGF对牙髓细胞迁移活性的影响。结果与对照组相比,bFGF在1.0～50.0ng/ml浓度下可促进牙髓细胞的增殖(P<0.05),bFGF最低显效浓度为1.0ng/ml,最佳显效浓度为10.0ng/ml。bFGF在1.0～50.0ng/ml浓度下诱导细胞迁移(P<0.05)。结论 bFGF可诱导体外培养人牙髓细胞的增殖和迁移,在牙髓牙本质复合体修复中可能发挥重要作用。

Objective To investigate the effect of basic fibroblast growth factor（bFGF） on the proliferation and migration of human dental pulp cells in vitro. Methods After being isolated from dental pulp tissue explants,the human dental pulp cells were divided into 5 groups randomly,and bFGF was added into each group until the ultimate concentrations were 1.0,5.0,10.0 and 50.0ng/ml respectively,while non-bFGF group served as control.The proliferation ability of dental pulp cells induced by bFGF at different concentrations was observed by absorbency A with CCK-8 assay.Meanwhile,the migration ability of dental pulp cells were examined by the number of cells migrating through micropores and staying on the outer bottom side of chamber systems with Transwell chamber assay. Results The proliferation of human dental pulp cells was increased when bFGF was used at the concentrations of 1.0～50.0ng/ml in comparison with that in the control cells（P0.05）,with its effect reached maximum at the concentration of 10.0ng/ml.Meanwhile,bFGF at the concentrations of 1.0～50.0ng/ml markedly induced the migration of dental pulp cells（P0.05）. Conclusion bFGF can promote the proliferation and migration of human dental pulp cells in vitro,and may play an important role in the reparation of pulpodentinal complex.