喷替酸锌钠对铅中毒小鼠的驱铅作用研究

Study of the Effect of Pentetate Zinc Trisodium on Excretion of Lead in Lead Poisoned Mice

目的研究喷替酸锌钠对慢性铅中毒小鼠体内铅的排泄效果,为临床用药提供实验依据。方法小鼠随机分为6组,连续10 d腹腔注射醋酸铅溶液,每日剂量为10 mg/kg,建立小鼠慢性铅中毒模型,末次染铅后24 h开始给药,连续7 d肌肉注射喷替酸锌钠或Ca-DTPA,喷替酸锌钠每日剂量分别为50、150、450 mg/kg,Ca-DTPA每日剂量为150 mg/kg。于注射开始后即每日收集尿液。停药后24 h处死动物,取血液、脑和双侧股骨,通过石墨炉原子吸收分光光度计法测小鼠血铅、脑铅、骨铅和7 d总的尿铅含量。结果喷替酸锌钠可显著降低染铅小鼠血、脑、骨骼中铅含量,明显增加尿铅含量。结论喷替酸锌钠对于慢性铅中毒小鼠体内的铅有明显的促排效果。

Objective To provide experimental evidence for clinical use of the drug by studying the effect of pentetate zinc trisodium（Zn-DTPA） on excretion of lead in chronic lead poisoned mice.Methods Mice were randomly divided into six groups.The lead poisoned mice model was established by injecting lead acetate intraperitoneally once daily for ten consecutive days at a daily dose of 10 mg/kg.Then pentetate zinc trisodium or pentetate calcium trisodium was administered intramuscularly to mice once daily for seven consecutive days Their urine was collected every day.The daily dose of pentetate zinc trisodium was 50,150 and 450 mg/kg respectively and the daily does of pentetate calcium trisodium was 150 mg/kg.Twenty-four hours after the final injection,the mice were sacrificed and the lead concentration in the blood,brain,femur and urine was determined by atomic absorption sepectrophotometer.Results Pentetate zinc trisodium could reduce lead concentration in the blood,brain and femur significantly and increase the content of lead in urine markedly.Conclusion Pentetate zinc trisodium can promote the excretion of lead in chronic lead-poisoned mice.