摘要
采用传统的水煮醇沉法从藏木香中提取可溶性多糖,通过Sevag法脱蛋白后,以葡萄糖为对照品,使用苯酚-硫酸法测定多糖含量.结果表明,在波长486nm处测定吸光度,10-100μg/mL范围内吸光度与被测含量之间具有良好的线性关系,藏木香中多糖的含量为64.37%.
The soluble polysaccharides in Inula helenium were extracted by the traditional method of boiling with water and depositing in alcohols.After removing proteins,the content of polysaccharides was determined by phenol-sulfuric acid method with glucose as standard sample.The result showed that there were favorable linearity relationship between the absorption and content determined in the range of 10-100μg/mL at the wavelength of 486nm.The content of polysaccharides was about 64.37%.
出处
《青海师范大学学报（自然科学版）》
2011年第1期35-37,共3页
Journal of Qinghai Normal University(Natural Science Edition)
关键词
藏木香
多糖
苯酚-硫酸法
inula helenium
polysaccharides
phenol-sulfuric acid method
