沙葱多糖的SephadexG—100凝胶柱纯化和分子量的测定

Studies on Purified with SephadexG—100 Column Chromatography and Molecular Weight Measured of Allium mongolium Regel Polysaccharides

对通过热水浸提、三氯乙酸除蛋白和活性炭脱色后的沙葱粗多糖,采用SephadexG—100凝胶柱层析纯化,收集合并单一峰溶液命名为SCSP,并对纯化产物SCSP进行相对分子质量的测定。结果表明,SCSP凝胶柱层析洗脱曲线为单一狭窄对称峰,说明为均一组分多糖;SCSP的相对分子质量为5.89×104克/摩尔。

To rough sand onion polysaccharides,which is extracted by hot water,trichloramine acetic except protein and activated carbon decoloring,adopting sand onion SephadexG—100 gel column chromatography purification,collect merger single submmit solution named SCSP,and the purification product SCSP for molecular weight determination.The results show that SCSP gel column chromatography elution curve for a single narrow symmetrical peak,explain for homogeneous components polysaccharide,SCSP relative molecular mass for 5.89x104.