五岁以下喘息儿童鼻咽分泌物和血清中白细胞介素-17测定的临床意义

Clinical significance of interleukin-17 in nasopharyngeal secretions and serum of wheezing children under 5 years old

目的 探讨5岁以下喘息儿童鼻咽分泌物（NPS）和血清中白细胞介素（IL）-17水平在喘息评估中的临床意义.方法 将53例5岁以下反复喘息患儿,分为有特应性体质组（喘息Ⅰ组,27例）和非特应性体质组（喘息Ⅱ组,26例）;并从同期住院治疗的疝气、肾结石等非感染性疾病术前患儿中随机抽取20例作为对照组.分别收集NPS和血清,经处理后用双抗体夹心酶联免疫吸附（ELISA）法检测IL-17水平.结果 喘息Ⅰ、Ⅱ组血清和NPS中IL-17水平分别为（1469.32±978.30）、（1473.70±974.35）ng/L和（263.34±131.80）、（788.28±132.40）ng/L,明显高于对照组的（36.48±2.00）、（36.45±5.00）ng/L（P＜0.01）,其中喘息Ⅰ组血清和NPS中IL-17水平高于喘息Ⅱ组（P＜0.05）;NPS和血清中IL-17呈正相关（r=0.313,P＜0.05）.结论 检测NPS中IL-17可以作为识别5岁以下有发展为哮喘倾向的喘息儿童的指标,并给予早期临床干预和治疗.

Objective To explore the clinical significance of interleukin （IL）- 17 in nasopharyngeal secretions （NPS） and serum of wheezing children under 5 years old. Methods Fifty-three children with recurrent wheezing under 5 years old were divided into wheezing group Ⅰ （with atopic high risks,27 cases）and wheezing group Ⅱ （without atopic high risks, 26 cases） ;20 children without infectious diseases such as hernia and renal calculus were enrolled as control group. After collecting and dealing their NPS and serum,the levels of IL-17 were measured by double-antibody sandwich enzyme-linked immunosorbent assay （ELISA）. Results The levels of IL-17 in serum and NPS in wheezing group Ⅰ [（1469.32 ± 978.30）,（1473.70 ±974.35） ng/L],and wheezing group Ⅱ [（263.34 ± 131.80）, （788.28±132.40） ng/L] were significantly higher than those in control group [（36.48 ± 2.00）, （36.45 ± 5.00） ng/L] （P 〈 0.01 ）, and the levels of IL- 17 in wheezing group Ⅰ were higher than those in wheezing group Ⅱ （P 〈 0.05 ）; the expression of IL- 17 in NPS and serum had a positive correlation （r = 0.313,P 〈 0.05）. Conclusion The detections of IL-17 in NPS can be used as laboratory index to distinguish wheezing children who have a tendency to persistent wheezing, and early intervention and treatment should be given.