高效液相色谱法分离双手性中心β-氨基酮旋光异构体

Resolution of optical isomers of β-amino ketone compounds with two chiral centres by high performance liquid chromatography

建立了使用AE.L ich rom AM1手性色谱柱(250mm×4.6mm,5μm)直接拆分含有双手性中心的β-氨基酮类化合物的4个旋光异构体的方法。考察了流动相的极性大小、洗脱方式及流速等因素对异构体拆分的影响,确定了分离β-氨基酮类化合物异构体的最佳分离条件:流动相为正己烷/异丙醇,洗脱模式为二元高压梯度洗脱,流速为1m L/m in。在上述色谱条件下,完成了4种含双手性中心的β-氨基酮类化合物的4个旋光异构体的基线分离(Rs>1.5)。结果表明该方法可快捷、简便地分离双手性中心β-氨基酮类化合物的4个旋光异构体。

A high performance liquid chromatographic method was developed for the resolution of optical isomers of β-amino ketone compounds containing two chiral centres.An AE.Lichrom AM 1 chiral chromatographic column（250 mm×4.6 mm,5 μm） was used.The influences of the mobile phase polarity,flow rate and elution mode were investigated.The optimum conditions were the use of hexane-isopropyl alcohol as the mobile phase with a flow rate of 1 mL/min and gradient elution.Under the above chromatographic conditions,the optical isomers of four kinds β-amino ketone compounds containing two chiral centres were baseline separated（Rs1.5）.It was proved that the method is fast and easy to separate the optical isomers of β-amino ketone compounds containing two chiral centres.