抗癌多肽A38对胃癌细胞SGC-7901凋亡的影响

Effect of anticancer peptide A38 on apoptosis in SGC-7901 human gastric cancer cells

目的:探讨经计算机辅助药物设计的抗癌多肽A38,或与顺铂联合应用对人胃癌细胞株SGC-7901的体外生长抑制作用及对肿瘤细胞早期凋亡的影响。方法:将人胃癌细胞株SGC-7901与不同浓度的A38、顺铂及A38+顺铂(DDP)分别进行体外培养,采用四甲基偶氮唑蓝法测定不同浓度的A38、顺铂及联合用药组在作用24,48,72 h后对SGC-7901细胞的生长抑制率;流式细胞仪分析细胞的凋亡率。结果:不同浓度A38、顺铂及联合用药后,SGC-7901细胞生长抑制率在不同浓度不同时段较对照组均显著增加(P均<0.01),联合用药组细胞生长抑制率较两单药组也明显增强(P均<0.01),呈现明显时效和量效关系。流式细胞仪检测凋亡结果显示:A38、顺铂及联合用药后,SGC-7901细胞的凋亡率在不同浓度不同时段较对照组均显著增加(P均<0.01),联合用药组细胞的凋亡率较两单药组也明显增加(P均<0.01)。结论:A38能抑制人胃癌细胞株SGC-7901细胞生长,诱导肿瘤细胞早期凋亡,小剂量顺铂与A38联用具有协同抑制作用,且效果比高浓度下的单用A38或单用顺铂的抑制作用明显。

Objective：To investigate the in vitro inhibitory effect of an anticancer peptide（A38,obtained by a computer-aided drug design） alone and in combination with cisplatin on early apoptosis of SGC-7901 cells,human gastric cancer cell line.Methods： Cultured cells were exposed to various concentrations of A38 or cisplatin alone,or A38 in combination with cisplatin for 24,48 and 72 h.The inhibition rate was determined by MTT assay.Cell apoptosis was analyzed by flow cytometry.Results： After treatment with A38 alone,cisplatin alone or A38 in combination with cisplatin,SGC-7901 cell growth was significantly increased as compared to control at various time-points and concentrations.Flow cytometry results showed a significant increase in early apoptosis rate at 24,48 and 72 h as well.The combination was more effective for both cell growth and apoptosis（P0.01）.Conclusion： A38 can inhibit growth of the human gastric cell line SGC-7901 cells and induce cell early apoptosis.A synergistic effect is achieved when low concentration cisplatin is combined with A38,showing a more significant inhibition than high concentrations of A38 alone or cisplatin alone.