多重耐药铜绿假单胞菌的耐药性分析

Resistance Analysis on Multidrug-resistant Pseudomonas Aeruginosa

[目的]探讨医院感染多重耐药铜绿假单胞菌的耐药性及耐药表型.[方法]对本院2009年10月至2010年10月临床检出菌,用天地人200B系统鉴定及药敏分析,并用三维法对分离的铜绿假单胞菌进行AmpC酶检测,同时用双纸片法进行超广谱β-内酰胺酶（ESBLs）检测.[结果]共检出铜绿假单胞菌64株,其中检出AmpC阳性菌株18株,产酶率为28.13%;产ESBLs菌株16株,产酶率为25.00%;产AmpC＋ESBLs菌株8株,产酶率为12.50%.在14种抗生素中,耐药率最低的是亚胺培南.[结论]住院病人免疫力低下的患者是铜绿假单胞菌的易感人群.亚胺培南是治疗感染多重耐药铜绿假单胞菌的有效药物.

[Objective] To explore the resistance and phenotypes of multidrug-resistant Pseudomonas aeruginosa in hospital, [Methods] The bacteria isolated from October 2009 to October 2010 in our hospital were identified by 200B system, and their drug susceptibility were analyzed. A three-dimensional method was used to detect AmpC enzyme in isolated Pseudomonas aeruginosa. A double disk test was used to detect extend-spectrum β-lactamases（ESBLs） in the isolated Pseudomonas aeruginosa, [Results] Totally 64 strains of Pseudomonas aeruginosa were detected. Among them, there were 18 AmpC-positive strains, 16 ESBLs strains and 8 AmpC＋ESBLs strains, and their producing enzyme rates were 28.13%, 25% and 12.5%, respectively. The resistant rate of imipenem（IPM） in the 14 antibiotics was the lowest. [Conclusion] The inpatients with low immunity are susceptible to infect Pseudomonas aeruginosa. IPM is effective for the treatment of multidrug-resistant Pseudomonas aeruginosa infection.