南阳油污土壤微环境分析及石油降解菌筛选

Analysis of Soil Micro-environment in Nanyang Oil Field and Screening of Oil Degrading Bacteria

测定了南阳油田石油污染土壤的一些理化性质,分析各种理化因子对其中的微生物的影响,并通过用以石油为单一碳源的培养基富集培养的方法从油污土壤中筛选降油菌株,还对它们的降油能力进行了评价。结果表明,南阳油田油污土壤的pH值、含水率等指标较有利于原油的降解,且南阳油田石油污染的土壤中的细菌数量与土壤的污染程度呈正相关。在这些油污土壤中共筛选到20株石油降解菌,其中NY-2、NY-7、和NY-17菌株的除油能力较强,它们的石油降解率分别达到47.8%、51.2%和46.3%。

Some physical and chemical properties of the polluted soil on Nanyang oil field were investigated,and the influence of the physical and chemical factors on the micro-organisms was analyzed.In addition,strains which had stronger degrading capacity were screened from oil polluted soil through the method of enrichment culture,using petroleum as the sole carbon source.The result indicated that the pH index and water content of the soil on Nanyang oil field was comparatively beneficial for the degradation of petroleum.The amount of bacteria in the polluted soil on Nanyang oil field was positively correlated with the degree of oil polution.Twenty kinds of hydrocarbon-degrading bacteria were isolated from the polluted soil.Among them,NY-2#,NY-7#,and the NY-17# strains had better degrading capacity,whose respective petroleum degradation rate achieved 47.8%,51.2% and 46.3% respectively.