钒(Ⅳ)-硫胺素荧光光度法的研究与应用

Fluorimetric Determination of Thiamine Based on the Thiamine-Vanadium Reaction

利用钒(Ⅳ)作为氧化剂将硫胺素氧化成硫胺荧,建立了一种测量痕量硫胺素的荧光分光光度法,并将该方法应用于药物中硫胺素的测定.该方法测定硫胺素的线性范围为0.8～240.0μg/L;检出限为0.45μg/L;对于40.0μg/L的硫胺素测定10次,其相对标准偏差为1.5%.

A fluorophotometric method for the determination of trace thiamine was studied. The methodwas based on the fluorimetric reaction of thiamine with vanadium(Ⅳ) .The linear range was 0.8～240.0μg/L and the limit of detection was 0. 45μG/L. The relative standard deviation was 1.5% for 10determinations of 40μg/L thiamine. The method has been applied to the determination of thiamine inpharmaceuticl preparations.