摘要
采用氯磺酸-吡啶法对米糠多糖(RBPS2 a)进行硫酸酯化修饰得到硫酸酯化米糠多糖(SRBPS2 a),使用HPGPC测定SRBPS2 a的分子质量,利用红外光谱和13CNMR进行结构分析,应用体内、外抗肿瘤试验对SRBPS2 a抗肿瘤活性进行评价。结果发现:SRBPS2 a的取代度为1.29,分子质量为3.5×105u,在体内和体外对小鼠乳腺癌细胞EMT-6有显著的抑制作用。RBPS2 a经硫酸酯化修饰后,SRBPS2 a主链结构保持-β(1→3)-D-甘露糖不变,侧链被切除,硫酸基团的取代发生在C-2和C-4位上,C-6氧化形成糖醛酸甲酯。
A novel sulfated polysaccharide SRBPS2a with anti -tumor activity was derived from defatted rice bran by chlorosulfonic acid - pyridine （CSA - Pyr） method. The average molecular weight of SRBPS2a was 3.5 × 10^5 u and the degree of sulfation （DS） was 1.29. The Fourier - Transform Infrared Spectra （FT - IR） and 13C NMR spectroscopy analysis revealed that SRBPS2a was mainly consist of β-（1→3） -D-galactopyranosyl residues, the sulfate substitution site was on C - 2 and C - 4 while the side chains were cut off during the sulfated reaction. Furthermore, SRBPS2a exhibited evident growth inhibition on mouse mammary tumor EMT -6 cells both in vitro and in vivo.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2011年第3期18-22,共5页
Food and Fermentation Industries
基金
公益性行业(农业)科研专项经费项目(200903043)
中央高校基本科研业务费专项资金资助(JUSRP11018)
江南大学青年预研基金(2009LQN21)
江南大学预研基金(2009LYY17)
关键词
脱脂米糠
酯化多糖
抗肿瘤活性
取代度
结构特性
defatted rice bran,sulfated polysaccbaride,anti-tumor activity,structural feature.
