纤维素酶对山葡萄渣中白藜芦醇提取的影响

Impact of Cellulase Enzymolysis on Extraction of Resveratrol from Amur Grape Residue

探讨了纤维素酶对山葡萄渣中白藜芦醇提取效果的影响,研究在单因素实验的基础上进行正交实验,考察酶浓度、pH、酶解温度、酶解时间对白藜芦醇含量的影响。结果表明,山葡萄渣白藜芦醇提取的最佳酶解条件为:酶浓度1.5 mg/g,pH 5.5,酶解温度55℃,酶解时间1.5h,在此条件下测得白藜芦醇的含量为0.680 mg/g。

In order to discuss the impact of cellulase on the extraction of resveratrol from amur grape residue,the research was investigated with the content of resveratrol as the guide and cellulose dosage, pH, temperature and enzymolysis time were determined. The enzymolysis conditions based on single factor experiment were optimized by orthogonal design. The optimum enzymolysis conditions were as follows： the cellulose dosage was 1.5 mg/g, the pH was 5.5, the temperature was 55℃ and the enzymolysis time was 1.5 h. Under these optimized conditions, the content of resveratrol was finalized at 0. 680 mg/g.